Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Jun 1;90(5):1000–1015. doi: 10.1016/j.neuron.2016.04.046

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2016 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Slow Anterograde Transport of Dynein in the Axon Is Dependent on Microtubules and an Interaction with Kinesin

(A) Kymographs of DMSO control and nocodazole-treated axons from dynein-GFP hippocampal neurons showing relative positions of anterograde and retrograde intercepts (blue) and the calculated midpoint displacement (green). Scale bar indicates anterograde direction.

(B) The mean relative position of the midpoint with time for DMSO and nocodazole-treated axons: n = 18 and 22 axons, respectively, from three independent primary cultures; solid lines, mean; ribbons, ±SEM.

(C) A linear regression was fitted to each axon’s midpoint displacement to find the velocity of displacement. The mean velocity (heavy line) ± SEM (box) is shown. Overlaid spots are the velocities for each measured kymograph with colors indicating overall direction of the kymograph.

(D) Kymographs of control and DIC1a peptide-treated axons from dynein-GFP hippocampal neurons showing relative positions of anterograde and retrograde intercepts (blue) and the calculated midpoint displacement (green).

(E) The mean relative position of the midpoint with time for control and DIC1a peptide-treated axons: n = 27 and 29 axons respectively from 3 independent primary cultures; solid lines, mean; ribbons, ±SEM.

(F) Results of linear regression on each axon’s midpoint displacement to find the velocity of displacement. The mean velocity (heavy line) ± SEM (box) is shown. Overlaid spots are the velocities for each measured kymograph with colors indicating the overall direction of the kymograph.