Skip to main content
. Author manuscript; available in PMC: 2017 Jun 2.
Published in final edited form as: Cell. 2016 Jun 2;165(6):1530–1545. doi: 10.1016/j.cell.2016.04.048

Figure 5. Altered gene expression in clonal isogenic deletions of rs737092, rs1175550, and rs1546723.

Figure 5

(A–C) Small clonal deletions were made in K562s across rs737092 (clones D1, D2), rs1175550 (clones D3, D4, D5), and rs1546723 (clones D6, D7) using CRISPR-Cas9 genome editing. Control clones were generated by transfecting Cas9 and pLKO.1 GFP constructs. Quantitative RT-PCR analysis of genes within a ~1 megabase wingspan of rs737092 (A), rs1175550 (B) and rs1546723 (C) and expressed over a minimum threshold (FPKM > 2, genes not meeting threshold are in grey) in HEPs was performed. A Bonferroni correction was applied at each locus. A red asterisk (*) indicates the position of the MFV.