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. Author manuscript; available in PMC: 2017 Jul 1.
Published in final edited form as: Neurobiol Aging. 2016 Mar 26;43:13–22. doi: 10.1016/j.neurobiolaging.2016.03.013

Figure 6.

Figure 6

Representative maximum intensity projections of z-stack images showing immunolabeled postsynaptic patches and the synaptic ribbon marker, CtBP2 in the utricular neuroepithelium of B6 (top row) and B6.CAST (middle row) mice of different age groups: B6 (A, B, C, D), B6.CAST (E, F, G, H). I. Hair cell specific LHX3 immunolabeling (blue) co-localized with CtBP2 nuclear staining (red). Inset in 6J shows the co-localization of pre-synaptic ribbons (red) with post-synaptic patches (green). Pre-adsorption of SHANK1a and CtBP2 antibodies with their respective blocking peptides showed no immunolabeling (K & L). (Red-CtBP2 - C-terminal Binding Protein 2; Green - SHANK1a - SH3 And Multiple Ankyrin Repeat Domains 1a; Blue- LHX3- LIM Homeobox 3), Scale bar = 5μm).