Table 1.

Primers used to amplify the ENG gene

EXON	PRIMERS		SIZE	Tª
	Forward 5’ → 3’	Reverse 5’ → 3’
1	ACTGGACACAGGATAAGGCCCAGC	AATACTTGGGGCCTGGTCCGTG	180 bp	62 °C
2	CACCTTATTCTCACCTGGCCTCTT	CTGCCTTGGAGCTTCCTCTGAG	249 bp	61 °C
3	GGGTGGCACAACCTATACAAAT	CAGAGATGGACAGTAGGGACCT	269 bp	60 °C
4	CTACATGGGATAGAGAGGGCAC	TTCCTCCTGAGCAGTATCATGAG	277 bp	55 °C
5	TGAGGGAAGGGACTGAGGTGC	GTGGGGACTAGTGTCAGGGGC	238 bp	63 °C
6	GGCCTGTCCGCTTCAGTGTT	GTTTTGTGTCCCGGGAGCTG	203 bp	58 °C
7	CCCCCTGTTCTGCCTCTCTC	CTGATCCAAGGGAGGGGAAG	256 bp	63 °C
8	ACACATATCACACAGTGACCAGC	CTAGGGGAGGAACCAGATGTC	253 bp	55 °C
9	CTCCTGATGGTGCCCCTCTCTTC	TTGTCTTGTGTTCTGAGCCCCTG	296 bp	60 °C
10	CTGCAGGGGCTCAGAACACA	GGCCAGGTGGGTTAGCACG	212 bp	61 °C
11	ATTGACCAAGTCTCCCTCCC	GAAAGGCGGAGAGGAAGTTC	211 bp	61 °C
12	GGTGGGGTGAAGAGCAGCTG	GACCTGGAAGCTCCCACTTGAA	359 bp	58 °C
13	GAGTAAACCTGGAAGCCGC	GCCACTAGAACAAACCCGAG	154 bp	55 °C
14 A	CCAGCACAACAGGGTAGGGGAT	CTCAGAGGCTTCACTGGGCTCC	255 bp	61 °C
14 B	AGGACCCTGACCTCCGCC	CTCTCCTGCTGGGCGAGC	198 bp	63 °C