Table 2.
Summary of parameters studied based on clinical staging distribution of sick dogs
| Clinical staging | Mean ± standard deviation | ||||||
|---|---|---|---|---|---|---|---|
| Number of dogs | IFN-γ LSA (pg/ml) | IFN-γ ConA (pg/ml) | IL-10 LSA (pg/ml) | IL-10 ConA (pg/ml) | PCR (parasite/ml) | ELISA (EU) | |
| I | 10 | 2022 ± 2687h | 4029 ± 3942i | 17 ± 18 | 82 ± 46 | 3.7 ± 10.4d | 46 ± 51a |
| IIa | 27 | 1909 ± 4373 | 7943 ± 7115j | 8 ± 14 | 57 ± 50 | 6 ± 10e | 5533 ± 11338b |
| IIb | 9 | 539 ± 1231 | 6777 ± 6068 | 7 ± 14 | 144 ± 130 | 101 ± 285f | 23,287 ± 27636 |
| III | 10 | 423 ± 629 | 8004 ± 7914k | 15 ± 19 | 94 ± 167 | 20 ± 26g | 12,416 ± 20,227c |
| IV | 4 | 94 ± 187 | 792 ± 350 | 5 ± 9 | 77 ± 112 | 6337 ± 12501 | 52,151 ± 44,365 |
| Total | 60 | 1354 ± 3220 | 6649 ± 6618 | 10 ± 15 | 83 ± 102 | 458 ± 3293 | 11,537 ± 22,240 |
Parameters under study were evaluated in relationship to clinical staging and letters from a to b correspond to statistically significant findings
Results for ELISA units aI < IIa, IIb, III, IV (Mann-Whitney U-test: Z = -3.7, P < 0.0001); bIIa > I (Mann-Whitney U-test: Z = -4.4, P < 0.0001) and IIa < IIb, IV (Mann-Whitney U-test: Z = -2.7, P = 0.002); cIII < IV (Mann-Whitney U-test: Z = -2.1, P = 0.036)
Results for PCR dI < IV (Mann-Whitney U-test: Z = -2.8, P < 0,002); eIIa < IV (Mann-Whitney U-test: Z = -3.1, P < 0.0001); fIIb < IV (Mann-Whitney U-test: Z = -2.2, P = 0.028); g III < IV (Mann-Whitney U-test: Z = -2.2, P = 0.024)
Results for IFN-γ LSA hI > IIb, III, IV (Mann-Whitney U-test: Z = -2.1, P < 0.035)
Results for IFN-γ ConA iI>IV (Mann-Whitney U-test: Z = -2.1, P = 0.036); jIIa > IV (Mann-Whitney U-test: Z = -2.7, P = 0.003); kIII > IV (Mann-Whitney U-test: Z = -2.5, P = 0.008)
Abbreviations: LSA L. infantum soluble antigen, ConA concavalin A