Table 3.
Summary of parameters based on IFN-γ producer versus IFN-γ non-producer dogs
| Clinical staging | IFN-γ producers (mean ± standard deviation) | IFN-γ non-producers (mean ± standard deviation) | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Number of dogs | IFN-γ LSA (pg/ml) | IL-10 LSA (pg/ml) | PCR (parasite/ml) | ELISA (EU) | Number of dogs | IFN-γ LSA (pg/ml) | IL-10 LSA (pg/ml) | PCR (parasite/ml) | ELISA (EU) | |
| I | 9 | 2247 ± 2749 | 18 ± 20 | 4.1 ± 11 | 50 ± 53 | 1 | 0 ± 0 | 15 ± 0 | 0 ± 0 | 13 ± 0 |
| IIa | 17 | 3031 ± 5240 | 12 ± 16 | 2 ± 4 | 2660 ± 7140 | 10 | 0 ± 0 | 3 ± 8 | 12 ± 14 | 10,418 ± 15,420 |
| IIb | 3 | 1616 ± 1860 | 14 ± 23 | 0.1 ± 0.1 | 14,373 ± 15,473 | 6 | 0 ± 0 | 3.3 ± 8 | 162 ± 360 | 27,744 ± 32,476 |
| III | 5 | 846 ± 665 | 29 ± 18 | 12 ± 19 | 7755 ± 5590 | 5 | 0 ± 0 | 0 ± 0 | 29 ± 31 | 17,077 ± 28,897 |
| IV | 1 | 375 ± 0 | 19 ± 0 | 35 ± 0 | 73,701 ± 0 | 3 | 0 ± 0 | 0 ± 0 | 8438 ± 14,419 | 44,967 ± 51,408 |
| Total | 35 | 2320 ± 3961a | 16 ± 18b | 5 ± 10 | 5750 ± 14,082 | 25 | 0 ± 0 | 2.4 ± 7 | 1100 ± 5112c | 19,638 ± 28,596d |
Statistical study was performed to compare IFN-γ producers versus non-IFN-γ producers. Letters a to d correspond to statistically significant differences
Results for IFN-γ LSA, IFN-γa producers > non-IFN-γ producers (Mann-Whitney U-test: Z = -6.7, P < 0.0001)
Results for IL-10 LSA, IFN-γb producers > non-IFN-γ producers (Mann-Whitney U-test: Z = -3.60, P < 0.0001)
Results for PCR, IFN-γc producers< non-IFN-γ producers (Mann-Whitney U-test: Z = -3.2, P = 0.001)
Results for ELISA units, IFN-γd producers< non-IFN-γ producers (Mann-Whitney U-test: Z = -3.2, P = 0.001)
Abbreviations: LSA L. infantum soluble antigen