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. 2016 Jun 3;15:94. doi: 10.1186/s12934-016-0494-7

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Engineering strategy for guanosine production. A deletion of the gene purA was combined with overexpression of the genes prs, purF, and guaB under the control of the strong P₄₃ promoter. The gene abbreviations are as follows: purA encodes adenylosuccinate synthase; prs encodes ribose-phosphate pyrophosphokinase; purF encodes amidophosphoribosyltransferase; guaB encodes IMP dehydrogenase