Table 1.
Primer sequences and amplification efficiency
| Target | Forward primer (5′–3′) | Reverse primer (5′–3′) | Amplicon (bp) | Reagent | Efficiency |
|---|---|---|---|---|---|
| GAPDH | ACCCACTCCTCCACCTTTGAC | TCCACCACCCTGTTGCTGTAG | 110 | ABI | 0.98 |
| TLR2 | GTGATAGGTGTGAGGCAGGT | GTGGCCGCCTTGATTCATAG | 136 | ABI | 0.93 |
| CD1c | TTTCTGCAGTTTCTGCTGCTA | GAGACGTGTTCCTGGGATG | 74 | ABI | 1.06 |
| CD54 | CCTTCCTCACCGTGTACTGG | AGCGTAGGGTAAGGTTCTTGC | 90 | ABI | 1.05 |
| CD68 | TTCCCCTATGGACACCTCAG | TTGTACTCCACCGCCATGTA | 86 | ABI | 1 |
| CCL4 | CCTGCTGCTTTTCTTACAC | CACAGACTTGCTTGCTTC | 126 | ABI | 1.09 |
| IL4 | GTCTCACCTCCCAACTGCTT | GTTACGGTCAACTCGGTGCA | 157 | Bio rad | 0.99 |
| IL4 | GTCTCACCTCCCAACTGCTT | GTTACGGTCAACTCGGTGCA | 157 | Roche | 1.01 |
| IL8 | GAAGGAACCATCTCACTG | CCACTCTCAATCACTCTC | 200 | Bio rad | 0.96 |
| IL8 | GAAGGAACCATCTCACTG | CCACTCTCAATCACTCTC | 200 | Roche | 0.94 |
| IL1RN | CCTGTCCTGTGTCAAGTCTGG | AGCGGATGAAGGCGAAGC | 110 | ABI | 0.93 |
| CEBPB | CGCTTACCTCGGCTACCA | ACGAGGAGGACGTGGAGAG | 65 | ABI | 0.94 |
| IL-18 | TGCATCAACTTTGTGGCAAT | ATAGAGGCCGATTTCCTTGG | 169 | ABI | 1 |
| V-FOS | ACTACCACTCACCCGCAGAC | CCAGGTCCGTGCAGAAGT | 75 | ABI | 0.98 |
| Egr-1 | AGCCCTACGAGCACCTGAC | GGTTTGGCTGGGGTAACTG | 92 | ABI | 0.9 |
| Egr-2 | TTGACCAGATGAACGGAGTG | TGGTTTCTAGGTGCAGAGACG | 121 | ABI | 0.92 |
| CD43 | AAGATGTCATCAGTGCCCCA | CACGGTGTGGGATCCTAGAG | 90 | ABI | 0.93 |
| CCR7 | GGTGGTGGCTCTCCTTGTC | ACTGTGGTGTTGTCTCCGATG | 84 | ABI | 1.1 |
| CD40 | GCAGGCACAAACAAGACTGA | ATGGCAAACAGGATCCCGAA | 95 | ABI | 0.91 |
| S01a | GGTACCGGGTCTCCACATGA | GGGAAAGTCACTCACCCAAGG | |||
| S01b | GTACCGGGTCTCCACCAGG | GGGAAAGTCACTCACCCAAGG | |||
| S03 | CTTTTGCTTTCTGTTTCTTAAGGGC | TCAATCTTTGGGCAGGTTGAA | |||
| S04a | CTGGTGCCCACAGTTACGCT | AAGGATGCGTGACTGCTATGG | |||
| S04b | CTGGTGCCCACAGTTACGCT | AGGATGCGTGACTGCTCCTC | |||
| S05a | AAAGTAGACACGGCCAGACTTAGG | CATCCCCACATACGGAAAAGA | |||
| S05b | AGTTAAAGTAGACACGGCCTCCC | CATCCCCACATACGGAAAAGA | |||
| S06 | CAGTCACCCCGTGAAGTCCT | TTTCCCCCATCTGCCTATTG | |||
| S07a | TGGTATTGGCTTTAAAATACTGGG | TGTACCCAAAACTCAGCTGCA | |||
| S07b | GGTATTGGCTTTAAAATACTCAACC | CAGCTGCAACAGTTATCAACGTT | |||
| S08a | CTGGATGCCTCACTGATCCA | TGGGAAGGATGCATATGATCTG | |||
| S08b | GCTGGATGCCTCACTGATGTT | TGGGAAGGATGCATATGATCTG | |||
| S09a | GGGCACCCGTGTGAGTTTT | TCAGCTTGTCTGCTTTCTGGAA | |||
| S09b | GGGCACCCGTGTGAGTTTT | CAGCTTGTCTGCTTTCTGCTG | |||
| S10a | GCCACAAGAGACTCAG | TGGCTTCCTTGAGGTGGAAT | |||
| S10b | TTAGAGCCACAAGAGACAACCAG | TGGCTTCCTTGAGGTGGAAT | |||
| S11a | TAGGATTCAACCCTGGAAGC | CCAGCATGCACCTGACTAACA | |||
| Hy | TTCTGGAACCTTTCTTTTCAGGC | ACTTCCCTCTGACATTACCTGATAATTG | |||
| HA-8p | TGCAGTCAGCAGATCACCC | CTTCTGGGCAACAGTTATGGA | |||
| KIR3 DS1 | CATCRgTTCCATgATgCg | CCACgATgTCCAggggA | |||
| TCCATCggTCCCATgATgTT |