. 2016 Jun 4;9:292. doi: 10.1186/s13104-016-2093-4

Table 2.

Prescribed PCR amplification program

Mix	Steps	Temperature °C	Duration	Cycles
ABI	UDG activation	50	2 min	Hold
	Activation	95	2 min	Hold
	Denature	95	15 s	40
	Anneal/extend	60	60 s	40
	Melt curve analysis	95	10 s
		60	60 s
		97	5 s
BioRad	Activation	95	3 min	Hold
	Denature	95	15 s	40
	Anneal/extend	60	45 s	40
	Melt curve analysis	95	10 s
		55	60 s
		95	15 s
Roche	Activation	95	10 min	Hold
	Denature	95	10 s	40
	Anneal	60	10 s
	Extend	72	10 s
	Melt curve analysis	95	10 s
		65	60 s
		95	15 s
General lab PCR program	Activation	95	10 min	Hold
	Denature	95	15 s	45
	Anneal/extend	60	60 s	45
	Melt curve analysis	95	10 s
		55	60 s
		97	5 s

The ramp of each machine were set to default