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. 2016 Jun 4;17:69. doi: 10.1186/s12931-016-0381-0

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Overview of the experimental study design. The antibody suspension bead array technology was used to generate protein profiles in both BAL and serum. This study was performed as a directed screening using 94 selected target proteins. Towards these, 96 antibodies were used for profiling of both the discovery and verification set of BAL and serum samples. Based on these results, four proteins were selected for further analysis and additional antibodies targeting these proteins were included. As a second verification, the set of 68 samples were analyzed using sandwich assays for two of the proteins