Fig. 3.

MiR-410 targets XIAP mRNA by direct binding to its 3′UTR. a Bioinformatics analysis identified complementarity of XIAP mRNA and the “seed sequence” of miR-410. b Luciferase activity of a reporter vector harboring the 3′UTR of XIAP (WT-wild type and MUT- the deletion mutant in the miR-410 recognition element) and c Real-time RT-PCR analysis for XIAP mRNA levels in TFK-1 cells transfected with miR-NC or miR-410 for 36 h. d Representative picture of Western blot analysis for XIAP in TFK-1 cells transfected with miR-NC or miR-410. e Real-time RT-PCR analysis for XIAP mRNA levels in EGI-1 cells transfected with miR-NC or miR-410 for 36 h