Table 1.
Quantitative data for retinal axonal projections to the suprachiasmatic nucleus (SCN), dorsal lateral geniculate nucleus (dLGN), ventral lateral geniculate nucleus (vLGN), medial terminal nucleus (MTN) and superior colliculus (SC)
| Structure | Measurement | W4-WT | AE1-WT | AF2-WT | Average | SD | T1-KO | W1-KO | AE2-KO | AF1-KO | Average | SD | KO vs WT; U-test statistics (2-tailed) |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| SCN | Volume contra proj (mm3) | 0.0145 | NA | 0.0130 | 0.0138 | 0.0011 | 0.0114 | 0.0169 | 0.0119 | 0.0132 | 0.0134 | 0.0025 | −2.9 %; NS (P = 0.64) |
| Volume ipsi proj (mm3) | 0.0129 | NA | 0.0125 | 0.0127 | 0.0003 | 0.0132 | 0.0119 | 0.0131 | 0.0116 | 0.0125 | 0.0008 | −1.6 %; NS (P = 1) | |
| dLGN | A-P length (max; μm) | 940 | 910 | 1040 | 963 | 68 | 930 | 1022 | 1050 | 1130 | 1033 | 83 | +7.3 %; NS (P = 0.29) |
| Lat extension (μm) | 900 | 780 | 720 | 800 | 92 | 750 | 780 | 720 | 750 | 750 | 24 | −6.3 %; NS (P = 0.47) | |
| Volume contra proj (mm3) | 0.1583 | 0.1284 | 0.1029 | 0.1299 | 0.0277 | 0.1146 | 0.1412 | 0.1389 | 0.1781 | 0.1432 | 0.0262 | +10 %; NS (P = 0.48) | |
| Volume ipsi proj (mm3) | 0.0207 | 0.0126 | 0.0161 | 0.0165 | 0.0041 | 0.0214 | 0.0168 | 0.0211 | 0.0139 | 0.0183 | 0.0036 | +11 %; NS (P = 0.29) | |
| Ratio ipsi/contra | 13.08 % | 9.81 % | 15.65 % | 12.85 % | 2.92 % | 18.67 % | 11.90 % | 15.19 % | 7.80 % | 13.39 % | 4.64 % | +4.2 %; NS (P = 0.16) | |
| vLGN | A-P length (max; μm) | 815 | 591 | 740 | 715 | 114 | 760 | 662 | 566 | 730 | 680 | 86 | −4.9 %; NS (P = 0.48) |
| Lat extension (μm) | 450 | 360 | 450 | 420 | 52 | 360 | 450 | 450 | 510 | 443 | 62 | +5.5 %; NS (P = 0.55) | |
| Volume contra proj (mm3) | 0.0398 | 0.0407 | 0.0418 | 0.0408 | 0.0010 | 0.0325 | 0.0353 | 0.0383 | 0.0464 | 0.0381 | 0.0060 | −7 %; NS (P = 0.29) | |
| Volume ipsi proj (mm3) | 0.00699 | 0.00651 | 0.00668 | 0.00673 | 0.00024 | 0.00656 | 0.00496 | 0.00631 | 0.00449 | 0.00558 | 0.00101 | −17 %; NS (P = 0.077) | |
| Ratio ipsi/contra | 17.56 % | 16.00 % | 15.98 % | 16.51 % | 0.91 % | 20.18 % | 14.05 % | 16.48 % | 9.68 % | 15.10 % | 4.41 % | −8.8 %; NS (P = 0.72) | |
| MTN | Volume contra proj (mm3) | 0.00912 | 0.00755 | 0.00755 | 0.00807 | 0.00091 | 0.0112 | 0.0105 | 0.0096 | 0.00915 | 0.01011 | 0.00092 | +25.3 %; S ( P = 0.034) |
| Volume ipsi proj (mm3) | none | none | none | none | none | none | none | ||||||
| SC | A-P length (max; μm) | 2100 | 1770 | 1930 | 1933 | 165 | 1765 | 1815 | 1680 | 1550 | 1703 | 116 | −12 %; NS (P = 0.077) |
| Lateral extension (μm) | 1500 | 1530 | 1590 | 1540 | 46 | 1650 | 1620 | 1530 | 1560 | 1590 | 55 | +3.2 %; NS (P = 0.21) | |
| Thickness contra proj (max; μm) | 250 | 255 | 270 | 258 | 10 | 195 | 215 | 210 | 220 | 210 | 11 | −19 %; S ( P = 0.032) | |
| Volume contra proj (mm3) | 0.526 | 0.454 | 0.504 | 0.495 | 0.037 | 0.315 | 0.414 | 0.302 | 0.321 | 0.338 | 0.051 | −32 %; S ( P = 0.034) | |
| Volume ipsi proj (mm3) | 0.00654 | 0.00726 | 0.00817 | 0.00732 | 0.00082 | 0.00127 | 0.00104 | 0.00088 | 0.00051 | 0.00093 | 0.00032 | −87 % (~8 fold smaller); S ( P = 0.034) | |
| Ratio ipsi/contra | 1.24 % | 1.60 % | 1.62 % | 1.49 % | 0.21 % | 0.40 % | 0.25 % | 0.29 % | 0.16 % | 0.28 % | 0.10 % | −81 % (~5.3 fold smaller); S ( P = 0.034) |
Abbreviations: ipsi ipsilateral, contra contralateral, proj projections, S significant; NS not significant, KO knockout, WT wild-type. Bold is used to indicate averages and standard deviations, as well as significant data in the last column