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. Author manuscript; available in PMC: 2016 Jun 4.
Published in final edited form as: Cancer Res. 2000 May 1;60(9):2411–2418.

Figure 1. Expression levels of loss-of-function BRCA1 mutants.

Figure 1

Cell lysates containing comparable cell numbers were separated on SDS-PAGE. At least two independent transformants were assayed for each mutant to control for clonal variation. LexA fusion mutant proteins expressed in yeast were detected by western blot using a monoclonal α-LexA antibody. A schematic representation of the fusion proteins bearing missense mutations (white arrow; M.W. 65 kDa) and a nonsense mutation (gray arrow; M.W. 49 kDa) is shown. Note that mutation Y1769X disrupts the BRCT-C but retains BRCT-N.