Table 2.
Transcriptional activity of insertion mutants.
| Pentapeptide Insertion |
Miller Unitsa | Probable secondary structure elementb |
|---|---|---|
| empty vector | 4.1 ± 3.0 | - |
| wild-type | 99.9 ± 14.1 | - |
| 1571SEGYP | 98.2 ± 98.2 | unknown |
| 1578PSGVP | 120.1 ± 52.6 | unknown |
| 1602PQGVP | 99.3 ± 15.9 | unknown |
| 1620DRGTP | 127.1 ± 11.0 | unknown |
| 1625NGVPH | 81.7 ± 8.2 | unknown |
| 1627MGVPP | 94.4 ± 6.8 | unknown |
| 1665stop | 1.6 ± 0.1 | α-helix 1 of BRCT-N |
| 1676RGTPL | 2.5 ± 0.2 | β-strand 2 of BRCT-N |
| 1678RGTPN | 0.7 ± 0.2 | β-strand 2 boundary of BRCT-N |
| 1695GVPQF | 4.3 ± 1.1 | β-strand 3/α-helix 2 loop of BRCT-N |
| 1709GGTPG | 1.0 ± 0.7 | α-helix 2/β-strand 4 loop of BRCT-N |
| 1717WGTPF | 2.1 ± 0.4 | α-helix 3 of BRCT-N |
| 1723RGTPI | 36.5 ± 15.0 | α-helix 3 boundary of BRCT-N |
| 1724GVPLK | 10.4 ± 2.5 | BRCT-N/BRCT-C interval |
| 1730GVPLN | 57.7 ± 7.2 | BRCT-N/BRCT-C interval |
| 1737GVPLR | 1.0 ± 0.5 | BRCT-N/BRCT-C interval |
| 1769GGYPY | 11.7 ± 11.1 | β-strand 1/α-helix 1 loop of BRCT-C |
| 1780GVPQL | 0.8 ± 0.3 | α-helix 1 of BRCT-C |
| 1793GVPLK | 372.9 ± 113.8 | β-strand 2/β-strand 3 turn of BRCT-C |
| 1822GVPLH | 5.7 ± 0.5 | α-helix 2 of BRCT-C |
| 1824GGTPI | 178.0 ± 34.4 | α-helix 2 boundary of BRCT-C |
a
Mutants in bold displayed activity equal to or higher than wild-type.
b
According to a BRCA1 BRCT model from ref. 36.