Table 3.

Transcriptional activity of human BRCA1 unclassified variants (aa 1560–1863).

Exon	Mutation	Activitya	Dogb	Mousec	Ratd	Nucleotidee	Base change	Probable secondary structure elementf	Reference
16	T1561I	+	A	T	T	4801	C to T	unknown	Durocher et al 1996 (41)
16	L1564P	+	L	L	P	4810	T to C	unknown	BICg
20	D1733G	+	D	E	E	5317	A to G	BRCT-N/BRCT-C interval	BIC
20	G1738E	−	G	G	G	5332	G to A	BRCT-N/BRCT-C interval	BIC
23	P1806A	+	P	P	L	5535	C to G	β-strand 2/β-strand 3 loop of BRCT-C	BIC

^aAt least ten independent clones were assayed and scored 8 hours after addition of X-gal. +: blue with same intensity as wild-type control. −: white, similar to two (F1761S; Y1769X) loss-of-function controls.

^bAmino acid correspond to predicted translation from canine Brca1 cDNA deposited in GenBank accession # U50709.

^cAmino acid correspond to predicted translation from murine Brca1 cDNA deposited in GenBank accession # U68174.

^dAmino acid correspond to predicted translation from rat Brca1 cDNA deposited in GenBank accession # AF036760.

^eNucleotide numbering corresponds to human BRCA1 cDNA deposited in GenBank accession #U14680.

^fAccording to a BRCA1 BRCT model from ref. 36.

^gBreast Cancer Information Core.