Fig. 2.

NRVM viability, organization, and adhesion rate on collagen and CNH-Col substrates. a NRVMs were stained with calcein-AM (green)/ethidium homodimer (red) on days 1 and 3 (bars = 100 um). The histogram shows that compared with the collagen group, the 0.15 and 0.2 mg/ml CNH-Col groups exhibited significant cytotoxicity to NRVMs on both days 1 and 3. b The CCK-8 assay shows significant differences between the collagen and CNH-Col substrates with 0.15 and 0.2 mg/ml CNHs. However, there was no significant difference between the collagen and CNH-Col substrates with 0.05 and 0.1 mg/ml CNHs. c Immunostaining of troponin T and sarcomeric α-actinin of NRVMs on days 3 and 7 (bars = 40 um). d There was a significant difference in the NRVM adhesion rate between the collagen and CNH-Col substrates with 0.05 and 0.1 mg/ml CNHs at both 12 and 24 h. Data are means ± SDs, n = 3