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. 2016 Jun 6;7:857. doi: 10.3389/fmicb.2016.00857

Figure 2.

Figure 2

CAAD possess an inherent membrane targeting ability. (A) Confocal fluorescent microscopy of Anabaena filaments expressing the LeuRS from Lyngbya sp. PCC 8106 fused to GFP (left), GFP (center), or the Anabaena ammonium transporter Amt1 fused to GFP (right). For each group of pictures, the left panel shows the fluorescence of GFP, the middle panel the red fluorescence of thylakoid membrane pigments and the right panel the merged picture of the former ones. (B) SDS-PAGE of fractionated extracts from E. coli C41(DE3) expressing Anabaena His-ValRSC (left) or His-ValRSΔC (right). CE, whole cell extracts; SF, soluble fraction; MF, membrane fraction. White arrowheads point to the position of the His-ValRSC or His-ValRSΔC. (C) Aminoacylation assay with membrane fractions from E. coli C41(DE3) cells expressing Anabaena His-ValRSC or His-ValRSΔC. A control of the activity of membranes from E. coli C41(DE3) carrying the pET28b vector is shown.