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. 2016 May 25;5(5):e51. doi: 10.1038/emi.2016.51

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Detection of HA2-specific serum antibodies from cross booster vaccination experiments by enzyme-linked immunosorbent assay (ELISA). The A/Texas/JMM_30/2012(H3N2) virus was used as a booster for trivalent HA2-PP. As a control, trivalent HA2-PP was used as a booster for both the subtype-specific virus and trivalent HA2-PP. Antiserum titers were determined by ELISA with HA2:76-130 peptide of H3 subtype. The highest reciprocal serum dilution that yielded absorbance >2-fold over the background value was taken as the ELISA end point titer. Titers are expressed as the mean±SD of six mice per group. *P<0.05, ***P<0.0005.