Table 1. Mouse immunization groups and challenge strains.
| Experiment | Group | Adjuvant | Dose |
Immunization scheme |
Challenge strain | |
|---|---|---|---|---|---|---|
| Prime | Boost | |||||
| Immunological experiments | Trivalent HA2-PP | Yes | 20 μg | Four immunizations | — | |
| WT-PP | Yes | 20 μg | Four immunizations | — | ||
| PBS control | Yes | — | Four immunizations | — | ||
| Linear-H1-HA2 control | Yes | 20 μg | Four immunizations | — | ||
| Linear-H3-HA2 control | Yes | 20 μg | Four immunizations | — | ||
| Linear-B-HA2 control | Yes | 20 μg | Four immunizations | — | ||
| H1-HA2-KLH control | Yes | 20 μg | Four immunizations | — | ||
| H3-HA2-KLH control | Yes | 20 μg | Four immunizations | — | ||
| B-HA2-KLH control | Yes | 20 μg | Four immunizations | — | ||
| H1 virus controla | No | 105 EID50 | Three immunizations | — | ||
| H3 virus controlb | No | 105 EID50 | Three immunizations | |||
| B virus controlc | No | 105 EID50 | Three immunizations | |||
| Cross booster vaccination experiments | Trivalent HA2-PP (prime/boost) | Yes | 20 μg | Trivalent HA2-PP | Trivalent HA2-PP | — |
| Virus (prime/boost)b | No | 105 EID50 | Virus | Virus | — | |
| Trivalent HA2-PP/ virus | Yes | 20 μg/105EID50 | Trivalent HA2-PP | Virus | — | |
| Virus/ trivalent HA2-PP | Yes | 105EID50/20 μg | Virus | Trivalent HA2-PP | — | |
| Virus challenge experiments | Trivalent HA2-PP | Yes | 20 μg | Three immunizations | H3N2b | |
| PBS | Yes | — | Three immunizations | H3N2b | ||
Abbreviations: Egg infective dose, EID50; keyhole limpet hemocyanin, KLH; phosphate-buffered saline, PBS; wild-type P particles, WT-PP.
a
A/17/California/2009/38(H1N1) virus.
b
A/Texas/JMM_30/2012(H3N2) virus.
c
B/56/Brisbane/60/80 virus.