Table 2.
Presence of genes potentially associated with biofilm formation, including cell wall anchored (CWA) proteinsa.
| Protein biofilm group |
PIA biofilm group |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Species |
S. lentus |
S. cohnii |
S. saprophyticus |
S. capitis |
S. epidermidis |
S. aureus |
||||
| Strain | MF1767 | MF1862 | MF1844 | MF4371 | MF6029 | MF1871 | MF1872 | MF1789 | ATCC 35984 | RN4220b |
| Locus tag prefix | AXY34_ | AXY37_ | AXY36_ | AXY40_ | AXY41_ | AXY38_ | AXY39_ | AXY35_ | SERP | SAOUHSC_b |
| PIA | – | – | – | – | – |
icaR icaADBC |
icaR icaADBC |
icaR icaADBC |
icaR icaADBC |
icaR icaADBC |
| Embp/Ebh | – | – | – | – | – | embpcd | embpd | embpd | embp | ebh |
| SasC/Mrp/FmtB domain proteins | – | – | – | – | – |
sesA sesG |
sesA sesGc |
sesA |
sesA sesG |
sasB sasC |
| Bap family | – | – | bapd | – | – | – | – | – | bhp | – |
| Serine-aspartate repeat proteins | clfB | clfB | – | – | – |
sdrXc sdrZLc |
sdrXc sdrZLc |
sdrEc sdrF sdrG sdrH |
sdrFd sdrG sdrH |
clfA clfB sdrC sdrD |
| FnBPA, FnBPB | – | – | – | – | – | – | – | – | – |
fnbA fnbB |
| S. saprophyticus surface protein G | – | – | – | sssGc | – | – | – | – | – | – |
| Uro-adherence factor A | – | – | – | uafAc | uafAc | – | – | – | – | – |
| S. lentus surface protein A | slsAc | slsAc | – | – | – | – | – | – | – | – |
| S. cohnii surface protein E | – | – | scsEc | – | – | – | – | – | – | – |
| S. epidermidis surface protein C | – | – | – | – | – | sesC | sesC | sesC | sesC | – |
| G5-E repeat family protein | – | – | – | – | – | – | – | – | aap | sasG |
| Three-helical bundle protein | – | – | – | – | – | spad | spad | – | – |
spa spi |
| SRRP proteins | – | 12885c | – | – | – | sraPc | sraPc | sraP | sraP | sraP |
| S. saprophyticus surface protein F | – | – | sssF | sssF | sssF | – | – | – | – | – |
| NEAT domain proteins |
isdC 10605 12645 |
isdC 06145 05890 |
– | – | – |
isdC 05010 04985 04990 |
isdC 11495 04030 04025 |
– | – |
isdC isdA isdB isdH |
| Other LPxTG domain proteins | – | – | 09850 09225 11360c |
– | 12070 |
sesB sesE-like sesH-like 10330 |
sesB sesE-like sesH-like 10295 |
sesB sesE sesH |
sesB sesE sesH sesI |
sasDd sasF sasH |
| Small basic protein (Sbp) | 09055 | 01990 | 08170 | 06645 | 04110 | 11465 | 05970 | 05425 | sbp | 00617 |
| Bifunctional autolysin AtlE | 06865c | 08990c | 03795c | aas | aasc | atlE | atlE | atlE | atlE | atl |
| Multifunctional autolysin Aae | 00485 | 01440 | 11620 | 10120 | 09065 | 07140 | 07640 | aae | aae | aaa |
| Eap/Emp | – | – | – | – | – | – | – | – | – |
eap emp |
aFor each identified gene, the gene name or locus tag is listed. The locus tag prefixes for each strain is listed below the strain names in the table header. Further details are found in Supplementary Table S2. bLocus tags are obtained from the genome of S. aureus NCTC 8325 (CP000253), from which S. aureus RN4220 is derived (Herbert et al., 2010), since the publicly available genome sequence of RN4220 (AFGU01000000) is not annotated. All listed genes were also present in RN4220. cThe predicted protein is encoded on multiple contigs. For further details, see Supplementary Table S2. dThe gene encodes a truncated protein and/or contains an internal stop codon.