Figure 1.

Nox machinery and ROS production in endothelial cells (EC), supernatant (SN), and endothelial microparticles (MP). (a) Expression of Nox1, Nox2, Nox4, p22^phox, p47^phox, and p67^phox was measured by Western blot analysis in EC, SN, and MP. MPs were isolated from the media of mouse aortic ECs. Blots are representative of at least four independent experiments. (b, c) ROS production by endothelial MPs as determined by lucigenin (b) and DHE/HPLC (c) in the presence and absence of the NADPH oxidase inhibitor apocynin (10 μM). ^∗ P < 0.01, MP versus SN, ⁺ P < 0.01 MP + apocynin versus MP, n = 3–5.