Table 1.
List of antibodies used.
| Antibody | Species | Supplier | Catalog number | Dilution factor |
|---|---|---|---|---|
| Nox1 | Goat | Santa Cruz Biotechnology | sc-5821 | 1 : 2000 |
| Nox2/gp91phox | Goat | Santa Cruz Biotechnology | sc-5827 | 1 : 1000 |
| Nox4 | Goat | Santa Cruz Biotechnology | sc-21860 | 1 : 1000 |
| p22phox | Rabbit | Santa Cruz Biotechnology | sc-20781 | 1 : 1000 |
| p47phox | Goat | Santa Cruz Biotechnology | sc-7660 | 1 : 1000 |
| p67phox | Rabbit | Abcam | ab109366 | 1 : 1000 |
| Phospho-ERK1/2 (Thr202/Tyr204) | Rabbit | Cell Signaling Technology | 9101S | 1 : 2000 |
| Total ERK1/2 | Rabbit | Cell Signaling Technology | 9102S | 1 : 2000 |
| Phospho-Src (Tyr416) | Rabbit | Cell Signaling Technology | 2101S | 1 : 2000 |
| Total Src | Rabbit | Cell Signaling Technology | 2108 | 1 : 2000 |
| Phospho-eNOS (Ser1177) | Rabbit | Cell Signaling Technology | 9571 | 1 : 1000 |
| Phospho-eNOS (Thr495) | Rabbit | Cell Signaling Technology | 9574 | 1 : 1000 |
| Total eNOS | Rabbit | Cell Signaling Technology | 9572 | 1 : 2000 |
| Phospho-AKT (Ser473) | Rabbit | Cell Signaling Technology | 9271 | 1 : 2000 |
| Total AKT | Rabbit | Cell Signaling Technology | 9272 | 1 : 2000 |
| Donkey anti-goat HRP | Donkey | Santa Cruz Biotechnology | sc-2033 | 1 : 2000 |
| Goat anti-rabbit HRP | Goat | Santa Cruz Biotechnology | sc-2030 | 1 : 2000 |
| Goat anti-mouse | Goat | Santa Cruz Biotechnology | sc-2031 | 1 : 2000 |