Table 1.
qRT-PCR primers in this study.
| Gene | Sequence (5′→3′) | GenBank accession | Product size (bp) |
|---|---|---|---|
| Col1α1 | GATCCTGCCGATGTCGCTAT (F) | NM_053304.1 | 276 |
| GGAGGTCTTGGTGGTTTTGTATTC (R) | |||
|
| |||
| Col3α1 | AAGGCTGAAGGAAATAG (F) | NM_032085.1 | 147 |
| AATGTCATAGGGTGCGATA (R) | |||
|
| |||
| ZO-1 | AACAGAGCCGAGCAGTTAGCC (F) | NM_001106266.1 | 238 |
| CAACATCAGCAATCGGTCCA (R) | |||
|
| |||
| E-cadherin | GTGCCACCACCAAAGATA (F) | NM_031334.1 | 195 |
| GGCTGAGACAACCCTAAT (R) | |||
|
| |||
| α-SMA | GGCATCCACGAAACCACCT (F) | NM_031004.2 | 212 |
| CCGCCGATCCAGACAGAAT (R) | |||
|
| |||
| BMP-7 | GTGGTCAACCCTCGGCACA (F) | NM_001191856.1 | 215 |
| GGCGTCTTGGAGCGATTCTG (R) | |||
|
| |||
| MIF | TCTCCGCCACCATGCCTATG (F) | NM_031051.1 | 178 |
| GGGTCGCTCGTGCCACTAAA (R) | |||
|
| |||
| β-actin | CCCATCTATGAGGGTTACGC (F) | NM_031144.2 | 150 |
| TTTAATGTCACGCACGATTTC (R) | |||