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. 2016 Jun 6;6:27315. doi: 10.1038/srep27315

Figure 1. pCS2 + -SINEUP-GFP activity in vitro.

Figure 1

HEK293T/17 were transfected with pEGFP-C2 and pCS2+/SINEUP-GFP constructs at 1:6 ratio (+SINEUP). Control cells were transfected with pEGFP-C2 and an empty control plasmid (-SINEUP). 48 hr after transfection, cells were lysed and processed for protein (a) and RNA (b) levels. Western blot was performed with anti-GFP antibody. β-actin was used as loading control. Fold-induction was calculated on Western blot images normalized to β-actin and relative to empty control samples. Expression of SINEUP-GFP (white bars) and quantity of GFP mRNA (grey bars) were monitored by Real Time PCR using specific primers. Data indicate mean ± SD. Data are representative of >3 independent replicas.