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. 2016 Jun 6;6:27149. doi: 10.1038/srep27149

Figure 3. The effect of IL-17A on collagen matrix production.

Figure 3

(A) Total collagen production assessed using Sircol assay. Data represents mean collagen production (μg/ml) ± SD in control and rhIL-17A(100 ng) treated normal hamstring tenocyte cultures over a 48 hour time course. *p < 0.05, **p < 0.01 compared to control samples.(Students t-test) (B) The levels of mRNA for Collagen type IA and Collagen type IIIA were determined by real time PCR over a 48 hour time (100 ng rhIL-17A). Data shown as the mean ± SD of triplicate samples and represent experiments on five individual normal hamstring tendon patient explant samples utilising GAPDH housekeeping versus control. *p < 0.05, **p < 0.01 (Students t-test) (C) Collagen I and III protein expression 24 hours post incubation in control versus 50/100 ng rhIL-17A conditions. Data are shown as the mean ± SD of triplicate samples and are in turn, representative of experiments performed on three individual normal hamstring tendon patient explants. *p < 0.05, **p < 0.01 compared to control samples (Students t-test). (D) Cultured normal hamstring tenocytes were incubated with recombinant IL-17A (100 ng) over 24 hours. Data shown represent levels of MCP-1, TNF-α, IL-8, IL-6 and MIP-1α in supernatants removed from culture at 24 hrs analysed using Luminex. Data shown are the mean ± SD of triplicate samples and are representative of five individual experiments *p < 0.05, **p < 0.01 (ANOVA).