Table 2. VLX1570 binding to immobilised 26S proteasome (VIVA Biosciences) analysed by surface plasmon resonance (SPR) using Biacore T200 and CM7 chip.

Immobilised protein on chip	Average protein load (RU)	VLX1570 binding Relative RU × 100 +/− S.D.
26S proteasome	8500	0.21 ± 0.09
26S proteasome Ub-VS	10850	0.04 ± 0.01

Protein load on chip and relative light units(RU)×100 ± SD (defined as average of the ratio of VLX1570 RU divided by individual protein load RU) are listed. Values are the average of two chip experiments repeated three times.