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. 2016 Jun 6;6:26064. doi: 10.1038/srep26064

Figure 7. JNK/Bcl-2/Beclin1 signaling pathway plays a key role in autophagic cell death induced by co-treatment of CUR and BBR.

Figure 7

(A) MCF-7 and MDA-MB-231 cells were treated with CUR (5 μM) and BBR (25 μM) alone and in combination for 6 h or 24 h. Protein extraction and Western blotting were performed to detect the levels of Beclin1, p-JNK and p-Bcl-2. GAPDH was used as the loading control. Cells co-treated with CUR (5 μM) and BBR (25 μM) with or without SP600125 (10 μM) pretreatment were subjected to measuring the levels of Beclin1, LC3-I, LC3-II, p-JNK and p-Bcl-2 by Western blotting (C), and determining the cell viability by MTT assay (B,D). Values represent the mean ± SD (n = 3). **p < 0.01.