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. 2016 Jun 6;6:27332. doi: 10.1038/srep27332

Figure 3. Blocking Rab7 inhibits Vac-V2 formation.

Figure 3

(A) Representative phase contrast images showing the opposite effects of Rab7 on Vac-V2 and Vac-Clc3 formation in HeLa cells. The arrows showed extremely large Vac-Clc3s that were induced by inhibiting Rab7. Scale bar, 20 μm. (B–D) The impacts of dominant negative Rab7 mutants, the constitutively active Rab7 (Q67L) mutant and shRab7 on the vacuole size, number of cells with vacuoles, and average vacuole number per cell, which were induced by Best3V2 or Clc3s as in (A). (B) Scatter plots showing the distribution of vacuole diameters induced by the indicated plasmids. (C) Scatter plots showing the average vacuole number per cell. (D) Scatter plots showing the percent of cells with vacuoles. One hundred cells were randomly selected for counting and calculation. (E) Vac-V2 formation in response to the inhibition of early endocytosis by the 4 dominant negative mutants, respectively. The error bars denote the S.D. values. The experiments were repeated 3 times.