Table 1. Inhibitory effects of trans-chalcone and its related compounds on F. trinervia and F. pringlei PEPC and their influences on the growth of three soil bacteria (C. glutamicum ATCC13032, Cg; P. putida KT2440, Pp; B. subtilis 168, Bs) and E. coli K-12 MG1655 (Ec).

IC₅₀ determination of chalcones on the C₃ and C₄ PEPCs using activity assays. The selectivity of chalcones on the C₄ PEPC over C₃ isoform is also indicated in the Table. Error bars represent standard errors of at least two independent measurements. The strains were cultured in a microscale cultivation system both in complex and minimal media in the presence of 0.1×, 1×, and 10× the IC₅₀ concentration determined for the F. trinervia PEP carboxylase. The Table indicates whether the final cell density (measured as backscatter at 620 nm) or the growth rate decreased (fbs↓ and μ↓, respectively), or the lag phase was extended (lag↑). All results refer to the highest chalcone concentration tested if not stated otherwise. Only effects leading to differences ≥10% compared to the untreated control cultures were included. Effects due to precipitation in the growth media occurring at 10× IC₅₀ of trans-chalcone and 2′-Hydroxychalcone were not included to this Table. Abbreviations: n.d., not determined; n.e., no effect on growth.