Table 1.

Specifications of the reaction conditions applied in the conjugation high yields of the final compound. All reactions were carried out for 16 h. For the coupling of NODAGA triethylamine (TEA) was used instead of DIPEA.

	DOTA	NOTA	DTPA	CHX-A''-DPTA	PCTA	Oxo-DO3A	NODAGA	CIM
Chelator precursor	DOTA-p-nitro-phenol ester	NOTA-difluor-phenol ester	DTPA-tetra (t-butyl ester)	CHX-A''-DTPA isothio-cyanate	PCTA-isothio-cyanate	Oxo-DO3A isothio-cyanate	NODAGA isothio-cyanate	CIM
Equivalents Chelator	4	2	2	3	2	2	2	2
Equivalents DIPEA	20	10	10	10	16	16	10 (TEA)	10
Equivalents HATU	-	-	1.96	-	-	-	-	1.90
Purification gradient	10-40% ACN	10-40% ACN	10-40% ACN	20-50% ACN	15-45% ACN	15-45% ACN	15-45% ACN	25-55% ACN