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. 2016 May 24;6(8):1205–1219. doi: 10.7150/thno.15083

Figure 2.

Figure 2

Axl activation is closely associated with EMT in breast cancer cells. (A-B) Whole-cell extracts from MCF-7 and MCF-7/ADR cells were processed with Human Phospho-Receptor Tyrosine Kinase Array Kit (R&D). The differential p-RTKs between MCF-7 and MCF-7/ADR cells were labeled and their relative activities of these RTKs between MCF-7 and MCF-7/ADR cells were shown graphically. (C) Western blot validation for phospho- and total Axl in MCF-7 and MCF-7/ADR cells. (D) Immunofluorescent staining for p-Axl in MCF-7 and MCF-7/ADR cells. (E) qRT-PCR indicated the upregulation of Gas6 mRNA in MCF-7/ADR cells. (F) Human breast cancer lines derived from mesenchymal group (n = 11) expressed high levels of Axl. (G) Levels of phospho- and total Axl in the indicated breast cancer cell lines by Western blotting. (H-J) Correlation between the level of Axl and EMT markers in a panel of 59 breast cancer cell lines. ***P<0.001.