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. 2016 May 24;6(8):1232–1243. doi: 10.7150/thno.14409

Figure 1.

Figure 1

EGFR TKIs trigger feedback activation of MAPK signaling in NSCLC cells. A. Western blot analysis of EGFR, MEK and ERK phosphorylation in PC9 cells in the presence of erlotinib (1 μM). B. Western blot analysis of EGFR and ERK phosphorylation in PC9 cells treated with afatinib (1 μM) or neratinib (1 μM). C. Western blot analysis of EGFR and ERK phosphorylation in PC9 cells treated with erlotinib (1 μM) in combination with trametinib (0.5 μM). D. Cell viability assay of PC9 cells treated with erlotinib (1 μM), trametinb (0.5 μM) or combination (Combo). *P<0.05, ANOVA followed by Tukey's post-test. E. Cell cycle analysis of PC9 cells upon treatment with DMSO or erlotinib (1 μM). F. PC9 cells were treated with erlotinib for 4 days. The remaining cells were seeded at different concentrations and exposed to erlotinib for totally 30 days. Cell numbers were quantified at day 30.