Figure 1. Generation of Sv2a^L174Q rats.

(A) SV2A structure showing the mutation site L174Q. Glycosylation sites in a long intravesicular loop and putative binding sites for levetiracetam are also indicated. (B) Sequence analysis for the point mutation of the Sv2a gene, illustrating the presence of a single nucleotide mutation T521A. (C) Alignment analysis of SV2A amino acid sequences in various vertebrate species and in the rat SV2 family.