Figure 3. Susceptibility of Sv2a^L174Q rats to PTZ-induced seizures and kindling.

(A) Left; typical time course (~4 min) of PTZ-induced excitatory behaviors and seizures evaluated by the time-sampling method (scored every 10 sec). Sv2a^L174Q and F344 rats were treated with an increasing dose of PTZ (10, 20 and 30 mg/kg, i.p., each 30 min interval) in a cumulative fashion. At 30 mg/kg (i.p.), PTZ induced clonic seizures in Sv2a^L174Q rats usually within 5 min after PTZ injection while PTZ showed no seizures in F344 rats. Right; Comparison of seizure intensity (maximal seizure score), total seizure duration and incidence rate. Each column represents the mean ± SEM or 7 rats. *p < 0.05, **p < 0.01, Significantly different from F344 rats. (B) Susceptibility to PTZ kindling following repeated treatments with a sub-convulsive dose (30 mg/kg/day) of PTZ. Left; Seizure intensity. Right; Seizure incidence rate (score of 3 or more). Each point represents the mean ± S.E.M. of 8 animals. **p < 0.01, Significantly different from F344 rats.