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. 2016 Jun 6;6:27290. doi: 10.1038/srep27290

Figure 2. Centrioles in Drosophila primary spermatocytes.

Figure 2

Asterless (Asl) localisation in an orthogonal centriole pair was detected with primary and secondary antibodies, the latter coupled to Alexa Fluor 488 for SIM, STED and SMLM. 100% available depletion laser power was used for STED. Scale bar, 0.5 μm. (a) Single optical section through the centre of the centrioles. (b) Measurements of the barrel widths of 30 centrioles per super-resolution technique. (c) FWHM measurements of 60 centriole walls per super-resolution technique. (d) Representative normalised line profiles for each super-resolution technique.