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. 2016 Jun 6;6:27290. doi: 10.1038/srep27290

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Copyright © 2016, Macmillan Publishers Limited

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Sec31A was detected with primary and secondary antibodies, the latter coupled to Alexa Fluor 488 for confocal imaging, SIM and STED and Alexa Fluor 647 for SMLM. 100% available depletion laser power was used for STED. (a,c) Overview of vesicles close to the nucleus in a single optical section. Boxed areas are magnified in (b,d). Scale bar, 5 μm. (b,d) Vesicles of various sizes with a little out of focus background visible as weak, small foci in STED. Scale bar, 0.5 μm.