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TGN46 distribution was detected with primary and secondary antibodies, the latter coupled to Alexa Fluor 488 for confocal imaging (a), SIM (b) and STED (c) and Alexa Fluor 647 for SMLM (d). 100% available depletion laser power was used for STED. Shown are single optical sections through part of the TGN. Scale bar, 5 µm. Boxed areas are magnified underneath. Scale bar, 1 µm.
