Table 1. Signal to noise ratios for the three super-resolution techniques.
Microtubules |
|||
---|---|---|---|
SIM | STED | SMLM | |
Mean signal ± standard deviation | 7820 ± 2850 | 8.2 ± 5.2 | 34687 ± 13329 |
Mean signal/standard deviation | 3.9 | 2.0 | 3.4 |
Mean signal/mean background | 54.1 | 11.7 | 2075 |
Total microtubule length (μm) | 72 | 48 | 70 |
Total background area (μm2) | 41 | 20 | 69 |
Centrioles | |||
SIM | STED | SMLM | |
Mean signal ± standard deviation | 26129 ± 7252 | 11.6 ± 2.5 | 21895 ± 3961 |
Mean signal/standard deviation | 4.2 | 2.8 | 2.4 |
Mean signal/mean background | 215 | 38.0 | 83.5 |
Total centriole wall length (μm) | 93 | 91 | 75 |
Total background area (μm2) | 130 | 89 | 65 |
The signal to noise ratio along the microtubules and centriole walls is given as mean signal/standard deviation. The signal to background noise ratio is given as mean signal/mean background.