Table 1. Overview of the 15 in silico predicted peptides and their corresponding ranking for intensity and equal distribution of transitions when spiked in a tryptic HCT116 digest.
Rank in digest | Peptide | Abbreviation | Selection set |
---|---|---|---|
1 | EAVSEILETSR | EAV | 2 |
2 | GLGASPGIGAGR | GLG | 3 |
3 | TIVVGSPNEK | TIV | 1 |
4 | NTIEGAEITPQK | NTI | 1 |
5 | VLGTPIEGIER | VLG | 1 |
6 | ALELENVLDR | ALE | 1 |
7 | AIETVDSAVR | AIE | 1 |
8 | IESIEEAIK | IES | 2 |
9 | GEIGEIPVVVEDR | GEI | 3 |
10 | GGGPALIGIGESDSNNR | GGG | 3 |
11 | AAAIVTDEGGR | AAA | 2 |
12 | VGNPAETLVSK | VGN | 3 |
13 | DLTSSIIQNENQHK | DLT | 3 |
14 | EIATAGTLEGR | EIA | 1 |
15 | GQDIAELEVR | GQD | 1 |
The last column depicts by which procedure the peptides were selected. The indicated abbreviation is the code the peptides were given during the experimental evaluation of the peptides.