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. 2016 Jun 6;6:27400. doi: 10.1038/srep27400

Figure 1. Developmental expression profile of synaptic proteins in in vivo VPA model.

Figure 1

(a) Decreased body weight in the offspring of in utero VPA exposed mice at P11 (n = 45 offspring from VPA dams, n = 33 offspring from vehicle treated control animals). (b) Developmental delay in eye opening in in utero VPA exposed mice (n = 45 offspring from VPA dams, n = 33 offspring from vehicle treated control animals). (c,d) Example western blots and quantification of relative expression level of synaptic proteins in somatosensory cortex of adult offspring (postnatal day 70–80) derived from VPA-treated dams (n = 35–43 animals total derived from 12–13 VPA dams, n = 32–40 animals from 15 control dams). Each data point indicates the mean expression level for animals from one dam. The bar indicates the mean of the individual data points across all dams. The % change in expression levels relative to data from vehicle treated control animals is displayed. Blots were cropped to display the relevant molecular weight range. Uncropped blots are presented in Fig. S4a. (e,f) Analysis as in (c,d) for adolescent VPA-offspring (postnatal day 15–21; n = 42–49 animals from 10–12 VPA dams, n = 44–51 animals from 9 control dams). Blots were cropped to display the relevant molecular weight range. Uncropped blots are presented in Fig. S4b.