Table 1. The primers used for quantitative RT-PCR’.
Forward primer sequence (5′ to 3′) | Reverse primer sequence (5′ to 3′) | |
---|---|---|
GAPDH | GTATGATTCCACCCACGGCA | GCCTTCTCCATGGTCGTGAA |
PRDM14 | AGTGGATGCTTCTCTGCTACC | TGCCTTTCTCTCTTGGTTCA |
PRDM1 | CAGTGCCGTGAAGTTTCCA | AAGGATGCCTCTGCCTGAAC |
SOX17 | CGCACGGAGTTTGAACAATA | CAGACGTCGGGGTAGTTACAG |
THBS | CCTGCGCAGCCTCTGATACC | AATGCGGTTGGAGCCACACA |
OTX2 | GTCGCGCCGCAAACTCCAAA | CCCGTTCCCTCCCAAGCAAT |
RAB15 | CCGCTTCACCGACAACGAGT | CCCTGGGCCCGTCGATAGTA |
MTL5 | AGTTGAAAGGGGGTACACAAATGCT | GGGGAAAGCTGACCCGTTGAC |
LSM2 | GCGGTGCAAGCACAATGCTC | GCTGGCAACTGCACATAGCG |
MMP2 | TATGGAAACGCCGACGGGGA | GGAACGGGAACTTGCAGGGC |
SMO | CCCATTCACTCCCGCACCAA | CTGCGAGAGCCAGACACGAG |
STELLA | CCCGCCTTTCAATCTGTCTCC | TCGCCGAACCGTGTATCGAA |
DAZL | GGGTCGCTTTGCTTATCCGC | TGCAGCAGACATTACTGCGA |
VASA | CCTGCCCAGGAATGCCATCA | ACTGGCCAACTTGGAGAATGGT |
STRA8 | TGGAGAAGGGAGCAACCCCA | ACCTGCCACTTTGAGGCTGT |
GSG2 | TTGGGGCAGATGGGAAGAGTA | ACTTCTTTCAGGCCGGTTGA |
TNP2 | CGGAGCTCAGGGCGAAAATAC | TCCCGTGTACAAGCTTTACTCAATG |
PRM2 | GACCAGGGCTGCAGACGGA | TACTCAAGATCTCGTGGGCTCCT |
OCT446 | CAAACTGAGGTGCCTGCCCTTC | ATTGAACTTCACCTTCCCTCCAACC |
SOX246 | CATCAACGGTACACTGCCTCTC | ACTCTCCTCCCATTTCCCTCTTT |
C-MYC46 | ATCCAAGACCACCACCACTG | GTTCACAGCAACATTCAGGTAGA |
pMXs-Oct420 | GACGGCATCGCAGCTTGGATACAC | GAGAAGGCGAAGTCGGAAG |