Figure 1. A promoter from the euo gene drives GFP expression in C. trachomatis.

(a) Map of transcription reporter vector pPvGFP::SW2. A core promoter from the C. trachomatis euo gene was cloned upstream of the tuf RBS region and the gfp gene. The unique restriction sites are indicated. Bar = 10 μm. (b) Appearance of living L2/pPvGFP::SW2 infected HeLa cells at 16 h pi. GFP-expressing inclusions (green) are shown with arrows. (c) Appearance of living L2/pBOMB-P_euo infected cells at 40 h pi. P_tet-controlled mCherry (red) was induced by adding αTC (20ng/ml) immediately after infection. Bar = 20 μm.