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. 2016 Jun 6;6:27244. doi: 10.1038/srep27244

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Copyright © 2016, Macmillan Publishers Limited

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(a) Time course of GFP expression in HeLa cells visualized by fluorescent microscopy. L2/pBOMB-P3 infected HeLa cells were imaged at the times indicated. The inset highlights GFP-expressing C. trachomatis organisms. Bar = 10 μM. (b) Flow cytometric data corroborate microscopic images in (a), showing changes in GFP expression in C. trachomatis infected HeLa cells. GFP-expressing cell population is indicated as percentage. The average mean fluorescence intensity (MFI) and the standard deviation at each time is shown. (c) One-step growth curve of C. trachomatis strains L2/434/Bu and L2/pBOMB-P3. (d) Isolated L2/pBOMB-P3 organisms, but not L2/pBOMBm, appear to be green-colored by direct observation.