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. 2016 Jun 6;6:27398. doi: 10.1038/srep27398

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(A) Colonies stained with anti-ck19 and -Ki67 antibodies. Scale bars: 200 μm. (B) Statistical analysis of the average number of cells in a colony on differently coated plates. (C) Percentage of proliferating ck19 + cells. Bars show the average of 3 independent experiments ± SD. Two-way ANOVA tests were used to assess differences between groups, *indicates a statistically significant (P < 0.01) difference.