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. 2016 Jun 6;6:27232. doi: 10.1038/srep27232

Figure 4. Tumor growth inhibition and cytokine production triggered by emulsified or non-emulsified mycobacteria.

Figure 4

High grade human T24 (a) and murine MB49 (b), and human low grade 5637 (c) BC cells were treated with mycobacteria in OO emulsion (white columns), in MO emulsion (gray columns) or in culture media (Non-E, black columns). Emulsions without bacteria or cell culture media were added to parallel wells (No-bact). In (a–c) results from the MTT assay are shown as the percentage of proliferation in relation to the control cells (Non-E No-bact). Data are expressed as the means ± SEMs of the cell culture technical triplicates of three independent experiments. IL-6 (d–f) and IL-8/KC (g–i) production by infected and non-infected (No-bact) BC cells. Results are shown as the means ± SEMs of two technical replicates of the cell culture supernatants. *p < 0.05; **p < 0.01; ***p < 0.001; &p < 0.05; &&p < 0.01; &&&p < 0.001 versus the respective control No-bact; #p < 0.05; ##p < 0.01 versus the respective BCG (Kruskal-Wallis H test followed by Mann-Whitney U test). OO, olive oil; MO, mineral oil; Non-E, mycobacteria in PBS-tween; No-Bact, preparations without mycobacteria.