Table-2.
Composition of TCF extender.
| Ingredients | Quantity |
|---|---|
| TCF buffer | 72 ml |
| Glycerol | 8 ml |
| Egg yolk | 20 ml |
| Penicillin G sodium | 80000 i.u |
| Streptomycin sulfate | 100 mg |
TCF=Tris-citric acid fructose
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