Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Jun 6;6:27459. doi: 10.1038/srep27459

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2016, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

Optimal methods for each type of cloning procedure have been selected (orange) for comparison with IVA (green). Labour time and requirements are shown for each example, with the universal IVA protocol significantly outperforming all methods, becoming the best option for all procedures. Of special importance are multisite applications (‘Complex Procedures’), where IVA halves the time required by the next best method and eliminates costs associated with enzymatic assembly and DNA. Furthermore, the IVA multi-site protocol surpasses optimal methods for performing single modifications (‘Simple Procedures’). All protocols require transformation into E. coli (grey). Contrasting with other methods, IVA only requires DpnI (‘Requirements’). (Phos. = phosphorylated).