Fig. 5.

Cdk5-mediated Foxo3 activation increases the promoter activities of Bim, FasL, MnSOD and APP. (A) Foxo3 promoter activity was determined in vector and p25-expressing cells *P<0.01, compared with untreated HT22 cells (Student's t-test). (B) Foxo3 promoter activity was determined in HT22 cells upon glutamate treatment with or without roscovitine and Cdk5 shRNA. *P<0.01, compared with untreated HT22 cells (Student's t-test). (C) Scrambled-shRNA-infected, Cdk5-shRNA- or Foxo3-shRNA-infected or roscovitine-exposed HT22 cells were treated with glutamate for 24 h. Cell viability was examined using an MTT assay. *P<0.01, compared with untreated HT22 cells (Student's t-test). (D) Bim promoter activity was determined in HT22 cells upon glutamate treatment. (E) FasL promoter activity was determined in HT22 cells upon glutamate treatment. *P<0.01, compared with untreated HT22 cells (Student's t-test). (F) MnSOD promoter activity was determined in HT22 cells upon glutamate treatment. *P<0.01, compared with untreated HT22 cells (Student's t-test). (G) APP promoter activity was determined in HT22 cells upon glutamate treatment. *P<0.01, compared with untreated HT22 cells (Student's t-test). (H) HT22 cells were treated with glutamate for 0–24 h and Bim levels analyzed. (I) HT22 cells were treated with glutamate for 18 h with or without Cdk5 shRNA or Foxo3 shRNA. The total level of Bim was analyzed. (J) HT22 cells were treated with glutamate similarly and MnSOD levels analyzed. (K) HT22 cells were treated with glutamate, with and without Foxo3 shRNA or Cdk5 shRNA, and MnSOD levels analyzed. Graphical results are mean±s.e.m. Each experiment was repeated at least three independent times.