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. Author manuscript; available in PMC: 2016 Jun 6.
Published in final edited form as: J Immunol. 2008 Dec 15;181(12):8534–8543. doi: 10.4049/jimmunol.181.12.8534

FIGURE 3.

FIGURE 3

Heterologous transfection of HeLa cells with Fc-Cx43 confers phagocytic ability. HeLa cells deficient in Cx43 were transfected with FcIIA and/or GFP and/or GFP-Cx43, then allowed to phagocytose SRBCs A, DIC micrograph of nontransfected HeLa cells in association with SRBCs (arrow). B and C, Confocal micrograph showing the GFP emission (B) and DIC image (C) revealing the distribution of SRBCs adherent to the surface of Fc-transfected HeLa cell. D and E, Confocal micrograph showing the GFP emission (D) and DIC image (E) of HeLa cells that had been cotransfected with FcRIIA and GFP-Cx43 and allowed to engulf SRBCs. Arrows show the presence of internalized SRBCs. Size bar = 10 μm. F, Phagocytosis capacity among the different groups. *, p < 0.05 vs control of at least seven separate experiments. G, SDS-PAGE with Abs to Cx43 in lysates obtained from RAW264.7 macrophages (Mθ, positive control), nontransfected HeLa cells (−ve), GFP-transfected HeLa cells (GFP), and GFP-Cx43-transfected HeLa cells (GFP-Cx43). Arrows show the location of Cx43 at 43 kDa in macrophages (the positive control) and GFP-Cx43 at 70 kDa in GFP-Cx43-transfected HeLa cells. Representative of three separate experiments.