Skip to main content
. Author manuscript; available in PMC: 2017 Jul 1.
Published in final edited form as: J Nutr Biochem. 2016 Apr 1;33:145–153. doi: 10.1016/j.jnutbio.2016.03.016

Figure 3.

Figure 3

Exposure to PCB 126 leads to sustained increases in plasma levels of TMAO after administration of dietary precursors. Mice were exposed to either vehicle oil or PCB 126 and 48 h later administered a dietary choline source. Plasma was collected at the indicated times for TMAO quantification (A) and levels were measured by HPLC/MS MS. For choline measurements (B), plasma was collected at the conclusion of the study and levels were measured by HPLC/MS MS. MRM transitions monitored included: TMAO-76/59.1, Choline-104.2/60.1, and d9-choline-113.1/69.1. AUC integration values were determined and all results were normalized to d9-choline internal standard values. Data are presented as mean±S.E.M. (*p<0.05, n=5 for each group, Student’s t-test).